The EZBioscience® 4× Reverse Transcription Master Mix is a ready-to-use premixed reagent that contains all the reagents necessary to synthesize first strand cDNA from total or poly(A)+ RNA using Reverse Transcriptase. Reaction products are applicable to subsequent PCR, qPCR and PCR cloning. The mix contains Reverse Transcriptase, RNase Inhibitor, optimized buffer system, dNTPs; Oligo dT18 and random primer as primer. The random primer in the Mix can help get better Ct values.
The Reverse Transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. The reverse transcriptase lacking RNase H activity is suitable for preparing full-length cDNA. And the multiple site-mutations of Reverse Transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. This product is also very suitable for reverse transcription using plant tissue RNA.
When using this kit, 4× RT Master Mix should be added to total RNA template, as recommended in the protocol, and add ddH2O to a total volume of 20 μl. Then, mix well and run the RT reaction. The RT reaction is recommended at 42°C for 15 minutes and 95°C for 30 seconds.
4× RT Master Mix
550 μl × 5 tubes
Nuclease free ddH2O
1 ml × 5 tubes
Store at -20°C.
1. Fast and simple procedure: 4× RT Master Mix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed in 20 minutes.
2. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.
For detailed information, please check the product manual.