Description
The EZBioscience® Color Reverse Transcription Kit is a new-generation reverse transcription kit with higher reverse transcription efficiency compared to previous generations, which contains high stability blue dyes.
The kit is a ready-to-use premixed reagent that contains all the components necessary to synthesize the first strand cDNA from total or poly(A)+ RNA using reverse transcriptase. Reaction products are applicable to subsequent PCR, qPCR. 4× RT Master Mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs, Oligo dT18 and Random Hexamer as primer. The random primer in the Mix can help get better Ct values.
The kit contains an inert blue dye in the 4× RT Master Mix of this Color Reverse Transcription Kit to avoid of pipetting errors. It can be used with the EZBioscience® Color SYBR Green qPCR Mix (A0012, A0012-R1, A0012-R2), which is supplemented with an inert red dye. Mixing cDNAs, primers and other components with the EZBioscience® Color SYBR Green qPCR Mix together in a qPCR reaction turns the solution into purple, which provides a visual aid when pipetting and decreases the risk of pipetting errors during reaction setup, especially when using white reaction vessels. And the dyes do not affect the specificity or sensitivity of qPCR reactions. So, these two kinds of kits are recommended to use together to get optimal results.
The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. The reverse transcriptase lacking RNase H activity is suitable for preparing full-length cDNA. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this reverse transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.
When using this kit, 4× RT Master Mix should be added to total RNA template, as recommended in the protocol, and add ddH2O to a total volume of 20 μl. Then, mix well and run the RT reaction.
Components
Components | A0010C (100 Rxns) | A0010C-L (500 Rxns) |
4× RT Master Mix | 550 μl | 550 μl × 5 tubes |
Nuclease free ddH2O | 1 ml | 1 ml × 5 tubes |
Storage
Store at -20°C.
Features
1. Fast and simple procedure: 4× RT Master Mix contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed in 20 minutes.
2. gDNA Remover can quickly and completely remove genome contamination, eliminating the influence of genomic DNA on the accuracy of qPCR results.
3. 4× RT Master Mix contains blue dye, which can be used with the EZBioscience® Color SYBR Green qPCR Mix (A0012, A0012-R1, A0012-R2), which is supplemented with an inert red dye. Mixing cDNAs, primers and other components with the EZBioscience® Color SYBR Green qPCR Mix together in a qPCR reaction turns the solution into purple, which provides a visual aid when pipetting and decreases the risk of pipetting errors during reaction setup, especially when using white reaction vessels.
4. Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, this kit reverses cDNA with the smallest quantitative CT value and has excellent reverse transcription efficiency.
5. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.
For detailed information, please check the product manual.
