Description
The EZBioscience® 4× Reverse Transcription Master Mix is a ready-to-use premixed reagent that contains all the reagents necessary to synthesize first strand cDNA from total or poly(A)+ RNA using Reverse Transcriptase. Reaction products are applicable to subsequent PCR, qPCR and PCR cloning. The mix contains Reverse Transcriptase, RNase Inhibitor, optimized buffer system, and dNTPs. Oligo dT18 and Random Hexamer are supplied in separate tubes as primer.
The Reverse Transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. The reverse transcriptase lacking RNase H activity is suitable for preparing full-length cDNA. And the multiple site-mutations of Reverse Transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. So more full-length cDNAs, and also, cDNAs as long as 20 kb can be synthesized. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. This product is also very suitable for reverse transcription using plant tissue RNA.
Components
Components | A0010T (100 Rxns) | A0010T-L (500 Rxns) |
4× RT Master Mix | 550 μl | 550 μl × 5 tubes |
Oligo dT18 (40 μM) | 110 μl | 110 μl × 5 tubes |
Random Hexamer (80 μM) | 110 μl | 110 μl × 5 tubes |
Nuclease free ddH2O | 1 ml | 1 ml × 5 tubes |
Storage
Store at -20°C.
Features
1. Oligo dT18 and Random Hexamer in this kit are packaged separately, so different types of reverse transcription primers can be used flexibly for different experimental designs.
2. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.
For detailed information, please check the product manual.
