Introduction
TheEZBioscience®4× EZscript Reverse Transcription Mix II (with gDNA Remover) is a new-generation fast reverse transcription kit with higher reverse transcription efficiency compared to previous generations.
The kit is suitable for real-time RT-PCR (RT-qPCR) that contains a gDNA Remover which can effectively eliminate the contamination of genomic DNA (gDNA) or other double stranded DNA during the analysis of gene expression. To accurately analyze gene expression, it is necessary to detect cDNA in samples without contaminating DNA. To avoid amplification of gDNA, primers can be designed on different exons spanning introns. However, there may be cases where a suitable primer cannot be designed, as with a gene with a single exon or a gene without a long intron. Also, it may be difficult to avoid unexpected amplification from gDNA due to non-specific amplification or the existence of pseudo-genes. Moreover, some labs are heavily contaminated by the PCR products of previous tests. This kit offers a potent gDNA Remover that can eliminate double stranded DNA in RNA sample in 5 minutes at room temperature without loss of RNA. Then the first strand of cDNA is synthesized by adding the 4× EZscript RT Mix II. Reaction products are applicable to subsequent PCR, qPCR.
The mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs, and Oligo dT18 and Random Hexamer as primers. The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.
Components
Components | EZB-RT2GQ (100 Rxns) | EZB-RT2GQ–L (500 Rxns) |
4× EZscript RT Mix II | 550 μl | 550 μl × 5 tubes |
gDNA Remover | 220 μl | 220 μl × 5 tubes |
Nuclease free ddH2O | 1 ml | 1 ml × 5 tubes |
Storage
Store at -20°C.
For detailed information, please check the product manual.
