The EZBioscience® 2× EZ Color SYBR Green qPCR Master Mix is a kit specifically designed for intercalator-based real-time PCR using SYBR Green I for detection. This kit premixed with a highly stable red dye can be combined with EZBioscience® Color Reverse Transcription Kit (Cat. No.: A0010C, A0010CG or A0010CGQ), Color All-in-one Reverse Transcription Kit (with DNase) (Cat. No.: RT3C, RT3CP), which contain blue dye. When performing a qPCR reaction, the cDNA (blue) generated by the Reverse Transcription Kit and the 2× EZ Color SYBR Green qPCR Master Mix (red) of this kit will turn the solution into purple, which provides a good visual aid when adding samples and greatly reduces the risk of sample addition errors. The Premix is supplied in 2× concentration and contains SYBR Green I, dNTPs, optimized buffer and a genetically engineered hot-start Taq DNA polymerase which uses an anti-Taq antibody, making it easy to prepare reaction mixtures. In addition, this kit contains 50× ROX Reference Dye 1 (ROX1) and 50× ROX Reference Dye 2 (ROX2), which are suitable for all qPCR instruments. This kit allows high amplification efficiency and high detection sensitivity in real-time PCR.
2× EZ Color SYBR Green qPCR Master Mix*1
50× ROX Reference Dye 1*2
220 µl × 5 tubes
50× ROX Reference Dye 2*2
220 µl × 5 tubes
*1: Contains hot-start DNA Polymerase with an anti-Taq antibody, dNTPs, Mg2+, and SYBR Green I and red dye.
*2: Used to rectify the error of fluorescence signals between different wells.
All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under the recommended storage conditions.
Please select an appropriate ROX according to the real-time PCR instrument used:
Do Not Use ROX
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™ 5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Roche LightCycler™ 96, Roche LightCycler™ 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.
Use ROX1 (1×)
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.
Use ROX2 (1×)
ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™ 7; Stratagene MX3000P™, MX3005P™, MX4000™.
For detailed information, please check the product manual.