The EZBioscience® EZ-Probe qPCR Master Mix for microRNA（ROX1 plus）uses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.
2× EZ-Probe qPCR Master Mix for microRNA (ROX1 plus)*
Probe (10 μM)
125 µl × 2 tubes
Universal 3’ qPCR Primer (10 μM)
U6 Primer (10 μM )
*: The ROX1 premixed is used to rectify the error of fluorescence signals between different wells.
All components should be stored at -20°C and avoid light and repeated thawing. The quality of this product is guaranteed for 12 months under recommended storage conditions.
Appropriate Real-time PCR instruments for this Kit are as follows:
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Analytikjena qTOWER 3G;
Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche LightCyclerTM 480; Thermo Scientific PikoReal Cycler.
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™
For detailed information, please check the product manual.