The EZBioscience® 2× EZ Color SYBR Green qPCR Master Mix (ROX1 plus) is a kit specifically designed for intercalator-based real-time PCR using SYBR Green I for detection. This kit premixed with a highly stable red dye can be combined with EZBioscience® Color Reverse Transcription Kit (Cat. No.: A0010C, A0010CG or A0010CGQ) and Color All-in-one Reverse Transcription Kit (with DNase) (Cat. No.: RT3C, RT3CP), which contain blue dye. When performing a qPCR reaction, the cDNA (blue) generated by the Reverse Transcription Kit and the 2× EZ Color SYBR Green qPCR Master Mix (ROX1 plus) (red) of this kit will turn the solution into purple, which provides a good visual aid when adding samples and greatly reduces the risk of sample addition errors. In addition, the Premix is supplied in 2× concentration and contains SYBR Green I, dNTPs, optimized buffer, high concentration of ROX Reference Dye (ROX1) and a genetically engineered hot-start Taq DNA polymerase which uses an anti-Taq antibody, making it easy to prepare reaction mixtures. This kit allows high amplification efficiency and high detection sensitivity in real-time PCR.
2× EZ Color SYBR Green qPCR Master Mix (ROX1 plus)*
*: Contains hot-start DNA Polymerase with an anti-Taq antibody, dNTPs, Mg2+, ROX1, SYBR Green I and red dye.
All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under recommended storage conditions.
Compatible instrument systems
Appropriate real-time PCR instruments for this kit are as follows:
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Roche LightCycler™ 96, Roche LightCycler™ 480; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G.
For detailed information, please check the product manual.