The EZBioscience® EZ-Probe qPCR Master Mix for microRNA uses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.
2× EZ-Probe qPCR Master Mix for microRNA
Probe (10 μM)
Universal 3’ qPCR Primer
U6 Primer (10 μM )
50× ROX Reference Dye 1*
50× ROX Reference Dye 2*
*: Used to rectify the error of fluorescence signals between different wells.
All components should be stored at -20°C and avoid light and repeated thawing. The Probe is suggested to divide into 3~4 parts, and store at -80°C and avoid light for long time storage. The quality of this product is guaranteed for 12 months under recommended storage conditions.
Please select an appropriate ROX according to the Real-time PCR instrument used:
Do Not Use ROX
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.
Use ROX 1 (1×)
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.
Use ROX 2 (1×)
ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™.
For detailed information, please check the product manual.