The EZBioscience® 2× Color SYBR Green qPCR Master Mix uses a specially modified Taq DNA polymerase protected by a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based Real-time quantitative PCR (qPCR). This Mix is supplemented with an inert red dye. And the EZBioscience® Color Reverse Transcription Kit (Cat. No.: A0010C, A0010CG or A0010CGQ) contains an inert blue dye to avoid of pipetting errors. Mixing cDNAs and other components together in a qPCR reaction turns the solution into purple. This provides a visual aid when pipetting and decreases the risk of pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR reactions. The mix is prepared at 2× reaction concentration, and can be directly used on both high and low-template qPCR with high sensitivity, specificity and reliability.
A0012 (500 Rxns)
2× Color SYBR Green qPCR Master Mix*1
5 ml (1 ml × 5 tubes)
50× ROX Reference Dye 1*2
50× ROX Reference Dye 2*2
*1: Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye and red dye.
*2: Used to rectify the error of fluorescence signals between different wells.
All components should be stored at -20°C and avoid of light. The quality of this product is guaranteed for 18 months under recommended storage conditions.
Please select an appropriate ROX according to the Real-time PCR instrument used:
Do Not Use ROX
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,
Opticon®, Opticon 2, Chromo4™;
Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche LightCyclerTM 480; Thermo Scientific PikoReal Cycler.
Use ROX 1 (1×)
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™
Use ROX 2 (1×)
ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, ViiA™7; Stratagene MX4000™, MX3000P™, MX3005P™
For detailed information, please check the product manual.