Description
The EZ-Press 96 RNA Purification Kit PLUS is an ideal reagent kit for simultaneously isolating 96 or 192 RNA samples from 2 × 104 to 5 × 105 human or animal cells. The EZ-Press 96 RNA Purification Kit PLUS provides an efficient and high-throughput solution for RNA sample preparation in areas such as drug screening, treatment monitoring, and basic research. In less than 1 hour, when processing two 96-well RNA plates in parallel, up to 192 high-purity RNA samples can be obtained (approximately 20 seconds per RNA sample).
The EZ-Press 96 RNA Purification Kit PLUS replaces current time-consuming and complex methods, including ethanol precipitation, extensive washing steps, or the use of toxic substances such as phenol and chloroform. The purified RNA can be used for various downstream applications. Including cDNA synthesis, RT-PCR, RT-qPCR, Northern, dot, and slot blot analysis, as well as primer extension, RNase/S1 nuclease protection, and gene chip detection. In addition, the EZ-Press 96 RNA Purification Kit PLUS can be used for purifying RNA from enzyme-catalyzed reactions, such as DNA digestion, protein digestion, RNA ligation, labeling reactions, etc.
Components
Components | EZ4002-S (2 × 96 Preps) | EZ4002-L (12 × 96 Preps) |
gDNA Removing 96 Plates*1 | 2 | 12 |
96-Well RNA Plates*1 | 2 | 12 |
Micro-pore Tape Sheets*1 | 6 | 36 |
Caps for Elution Plates*1 | 2 | 12 |
96-Well Collection Plates (Square Hole) *1 | 4 | 12 × 2 |
96-Well Elution Plates (Round Hole) *1 | 2 | 12 |
Buffer RLB | 35 ml | 200 ml |
Buffer RW1*2 | 70 ml | 210 ml × 2 |
Buffer RW2*2 | 30 ml | 90 ml × 2 |
Elution Buffer*3 | 30 ml | 100 ml × 2 |
Note: *1. For the sake of simplicity, these 6 devices will be referred to as DNA clearance well plate, RNA extraction well plate, breathable sealing film/sealing film, plate lid, collection well plate, and elution well plate in this article. *2. Before using Buffer RW1 and Buffer RW2 for the first time, it is necessary to add the volume of anhydrous ethanol indicated on the bottle label (the volume ratio of Buffer RW1 to anhydrous ethanol is 1:1, and the volume ratio of Buffer RW2 to anhydrous ethanol is 1:4), and mix thoroughly before use. *3. The Elution Buffer in this reagent kit is Nuclease-free ddH2O.
Storage
The gDNA Removing 96 Plates in this product should be stored at 2 ~ 8°C, while all other components should be stored at room temperature and can be stably stored for 12 months. See the expiration date on the product label for details.
Notice
1. Before using Buffer RW1 and Buffer RW2 for the first time, it is necessary to add the volume of anhydrous ethanol indicated on the bottle label (the volume ratio of Buffer RW1 to anhydrous ethanol is 1:1, and the volume ratio of Buffer RW2 to anhydrous ethanol is 1:4), and mix thoroughly before use.
2. Regarding centrifugation: During the DNA removal step, it is necessary to place the gDNA Removing 96 Plates (hereinafter referred to as " DNA clearance plate ") on the 96-Well Collection Plates (hereinafter referred to as " collection plate "). After adding the liquid, cover the DNA cleanup plate with a Micro-pore Tape Sheet (hereinafter referred to as "breathable sealing film/sealing film") before centrifugation; during the RNA extraction and purification steps, place the 96-Well RNA Plates (hereinafter referred to as " RNA extraction plate ") on the collection plate. After adding the liquid, cover the RNA extraction plate with a breathable sealing film before centrifugation. During the RNA elution step, the RNA extraction plate needs to be placed on the 96-Well Elution Plates (referred to as the elution plates below). After adding the liquid and before centrifugation, cover the RNA extraction plate with a breathable sealing film. The collection well plate needs to be reused during the cleaning stage, and the elution plate should be used during the elution stage.
3. The recommended range for the cell counts of each sample is 2 × 104 to 5 × 105.
4. For your safety and health, please wear a lab coat, disposable latex gloves, and disposable masks.
Materials
Tabletop centrifuge with swing-out rotor for deep-well plates at a speed of 2100 g or higher, anhydrous ethanol, horizontal shaker, etc.
For detailed information, please check the product manual.
