Introduction
The EZBioscience® EZ-Probe qPCR Master Mix for microRNA uses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.
Components
Components | EZB-miProbe-S (200 Rxns) | EZB-miProbe (500 Rxns) |
2× EZ-Probe qPCR Master Mix for microRNA | 2 ml | 5 ml |
Probe (10 μM) | 45 µl | 110 μl |
Universal 3’ qPCR Primer (10 μM) | 100 μl | 250 μl |
U6 Primer (10 μM ) | 50 μl | 125 μl |
50× ROX Reference Dye 1* | 100 µl | 220 µl |
50× ROX Reference Dye 2* | 100 µl | 220 µl |
*: Used to rectify the error of fluorescence signals between different wells.
Storage
All components should be stored at -20°C and avoid light and repeated thawing. The Probe is suggested to divide into 3~4 parts, and store at -80°C and avoid light for long time storage. The quality of this product is guaranteed for 12 months under recommended storage conditions.
Notice
Please select an appropriate ROX according to the Real-time PCR instrument used:
Do Not Use ROX | Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®. |
Use ROX 1 (1×) | ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™. |
Use ROX 2 (1×) | ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™. |
For detailed information, please check the product manual.
