EZ-Probe qPCR Master Mix for microRNA (ROX1 plus) (EZB-miProbe-R1)

EZ-Probe qPCR Master Mix for microRNA (ROX1 plus) (EZB-miProbe-R1)

Type:     miRNA RT-qPCR

Catalog Number:    EZB-miProbe-R1-S/EZB-miProbe-R1

Pack Size:    200/500 Rxns

Price:    250/500 USD

Promotion:    180/350 USD (22 Customers Bought)


The EZBioscience® EZ-Probe qPCR Master Mix for microRNAROX1 plususes a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.




(200 Rxns)


(500 Rxns)

2× EZ-Probe qPCR Master Mix for microRNA (ROX1 plus)*

2 ml

5 ml

Probe   (10 μM)

100 µl

125 µl × 2 tubes

Universal 3’ qPCR Primer (10 μM)

100 μl

250 μl

U6 Primer (10 μM )

50 μl

125 μl

*: The ROX1 premixed is used to rectify the error of fluorescence signals between different wells.


All components should be stored at -20°C and avoid light and repeated thawing. The quality of this product is guaranteed for 12 months under recommended storage conditions.


Appropriate Real-time PCR instruments for this Kit are as follows:

Bio-Rad CFX96™, CFX384™, iCycler iQ™,   iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Analytikjena   qTOWER 3G;

Eppendorf Mastercycler® ep realplex,   realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene®   3000, Rotor-Gene® 6000; Roche LightCyclerTM 480; Thermo Scientific PikoReal   Cycler.

ABI 5700, 7000, 7300, 7700,   7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™

For detailed information, please check the product manual.