Introduction
The EZBioscience® EZ-Probe qPCR Master Mix for microRNA（ROX1 plus）uses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.
Components
Components | EZB-miProbe-R1-S (200 Rxns) | EZB-miProbe-R1 (500 Rxns) |
2× EZ-Probe qPCR Master Mix for microRNA (ROX1 plus)* | 2 ml | 5 ml |
Probe (10 μM) | 90 µl | 220 µl |
Universal 3’ qPCR Primer (10 μM) | 200 μl | 500 μl |
U6 Primer (10 μM ) | 100 μl | 250 μl |
*: The ROX1 premixed is used to rectify the error of fluorescence signals between different wells.
Storage
All components should be stored at -20°C and avoid light and repeated thawing. The quality of this product is guaranteed for 12 months under recommended storage conditions.
Notice
Appropriate Real-time PCR instruments for this Kit are as follows:
Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Cepheid SmartCycler®; Analytikjena qTOWER 3G; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™ |
For detailed information, please check the product manual.
