Introduction
The EZBioscience® EZ-press SINGLE Cell to cDNA Kit allows researchers to analyze the gene expression of single or very low cell numbers (1 ~ 1,000 cells) in a reliable, sensitive and easy way. Cells are first lysed by the unique Cell Lysis Buffer. Then cDNA is produced and pre-amplified using gene specific primers from the cell lysate in the same tube. Due to the pre-amplification following reverse transcription, extremely low concentration of RNA from a very small amount of cells can be detected by the subsequent qPCR.
Components
Components | B0011 (100 Rxns) |
Cell Lysis Buffer | 5.5 ml |
2× Pre-Amp Mix | 1.1 ml |
Nuclease free ddH2O | 1 ml |
Storage
Store at -20°C.
Experimental Procedure at a Glance
For detailed information, please check the product manual.
