2× EZ Color SYBR Green qPCR Master Mix (CQ23)

2× EZ Color SYBR Green qPCR Master Mix (CQ23)

  • Type:     Realtime-PCR Reagents

  • Catalog Number:    CQ23

  • Pack Size:    5ml

  • Price:    300 USD

  • Promotion:    200 USD (261 Customers Bought)


The EZBioscience® 2× EZ Color SYBR Green qPCR Master Mix is a kit specifically designed for intercalator-based real-time PCR using SYBR Green I for detection. This kit premixed with a highly stable red dye can be combined with EZBioscience® Color Reverse Transcription Kit (Cat. No.: A0010C, A0010CG or A0010CGQ), Color All-in-one Reverse Transcription Kit (with DNase) (Cat. No.: RT3C, RT3CP), which contain blue dye. When performing a qPCR reaction, the cDNA (blue) generated by the Reverse Transcription Kit and the 2× EZ Color SYBR Green qPCR Master Mix (red) of this kit will turn the solution into purple, which provides a good visual aid when adding samples and greatly reduces the risk of sample addition errors. The Premix is supplied in  concentration and contains SYBR Green I, dNTPs, optimized buffer and a genetically engineered hot-start Taq DNA polymerase which uses an anti-Taq antibody, making it easy to prepare reaction mixtures. In addition, this kit contains 50× ROX Reference Dye 1 (ROX1) and 50× ROX Reference Dye 2 (ROX2), which are suitable for all qPCR instruments. This kit allows high amplification efficiency and high detection sensitivity in real-time PCR. 





2× EZ Color SYBR Green qPCR Master Mix*1

5 ml

25 ml

50× ROX Reference Dye 1*2

220 µl

220 µl × 5 tubes

50× ROX Reference Dye 2*2

220 µl

220 µl × 5 tubes

*1: Contains hot-start DNA Polymerase with an anti-Taq antibody, dNTPs, Mg2+, and SYBR Green I and red dye.

*2: Used to rectify the error of fluorescence signals between different wells.


All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under the recommended storage conditions.


Please select an appropriate ROX according to the real-time PCR instrument used:

Do Not Use ROX


Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™ 5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Roche LightCycler™ 96, Roche LightCycler™ 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.

Use ROX1 ()

ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.

Use ROX2 ()

ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™ 7; Stratagene MX3000P™, MX3005P™, MX4000™.

For detailed information, please check the product manual.