Introduction
The EZBioscience® 2× qPCR mix for microRNA (ROX1 plus) uses a specially modified Taq DNA polymerase protected by a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based Real-time quantitative PCR (qPCR) for microRNA expression detection.
The mix is prepared at 2× reaction concentration, and is specially optimized for microRNA, which strikingly improves the microRNA amplification and reduces the non-specific amplification.
Meanwhile, the ROX1 passive reference dye (high ROX) is premixed in the mixture to make it an excellent ready to use qPCR reagent for microRNA expression detection. Besides, a universal 3’ qPCR primer is also supplied in this Kit.
It is recommended that the kit be used together with the EZBioscience® EZ-press Cell to cDNA Kit PLUS for microRNA, or microRNA Reverse Transcription Kit (Cat. No.: EZB-miRT1 or EZB-miRT2).
Components
Components | EZB-miQP1-S (200Rxns) | EZB-miQP1-L (500Rxns) |
2× qPCR mix for microRNA (ROX1 plus)* | 2 ml (1 ml*2 tubes) | 5 ml (1 ml*5 tubes) |
Universal 3’ qPCR Primer (10 μM) | 100 μl | 250 μl |
U6 Primer (10 μM ) | 50 μl | 125 μl |
*: Contain Hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye, and premixed with and ROX1, to rectify the error of fluorescence signals between different wells.
Storage
Store at -20°C.
For detailed information, please check the product manual.
