Exosomes are small vesicles (30–120 nm) containing RNA and protein that are secreted by various types of cells in culture, and found in abundance in body fluids, such as blood, saliva, urine, and breast milk. Exosomes were reported to function as intercellular messengers, delivering their cargo of effector or signaling macromolecules between specific cells; however, their formation, the makeup of the cargo, and biological pathways in which they are involved remain unclear.
The biological & medical study of exosome function and trafficking requires the isolation of intact exosomes, but the current methods used are tedious and difficult. The EZBioscience Exosome Isolation Kit (from cell culture media) provides a simple and reliable method of concentrating intact exosomes from cell culture media samples. By tying up water molecules, the Exosome Isolation reagent forces less-soluble components (i.e. exosomes) out of solution, allowing them to be collected with a brief and relatively low-speed centrifugation.
After Isolation, the exosome can be used for further experiments, auch as Immuno-blotting and RNA purification, RNA sequencing, and RT-qPCR etc. .
Note: The purified exosome sample may be stored at 2 ~ 8°C for up to 7 days or can be stored below -80°C for long term storage. To minimize the risk of RNase contamination, we recommend proceeding directly with further downstream sample processing.
For detailed information, please check the product manual.
