EZ-press Tissue Direct PCR Kit（EZB-TDP1）

Introduction

The EZBioscience^® EZ-press Tissue Direct PCR Kit is specially used for mouse genotyping, including DNA extraction and PCR amplification system. This kit can quickly release complete genomic DNA from mouse ears, tails, toes, and other tissues without the need for homogenization, overnight digestion, phenol-chloroform extraction, DNA precipitation, or column purification, which greatly shortens the experimental operation time. During use, directly immerse the tissue into Lysis Buffer premixed with Proteinase Mix, incubate at 55°C for 15 minutes to lysis tissue, and then heat at 95°C for 5 minutes to inactivate Proteinase. After centrifugation, the supernatant of the lysate can be directly used as a template for PCR amplification.

The 2x Direct-PCR Mix (Dye plus) in this kit contains DNA Taq Polymerase, dNTPs and a highly optimized buffer system with superior amplification and anti-interference ability. In the PCR reaction, only the template and primers need to be added to carry out the amplification, which reduces operations such as pipe opening and pipetting, and significantly improves the detection throughput and reproducibility of results. The dye is also premixed in the mix, and loaded directly after the PCR reaction. The PCR product has a base A at the 3' end and can be directly cloned into a T vector. This kit has a wide range of applications, and is especially suitable for mouse genotyping, transgenic detection and gene knockout analysis.

Components

Storage

Store the Lysis Buffer at 2 ~ 8°C and protected from light. Store other components of this product at -20°C.

Application

Apply to mouse genotyping, mouse transgene detection, and mouse gene knockout analysis.

Notice

1. The step of Proteinase K inactivation (95°C for 5 min) must be performed, otherwise its residual activity will inhibit the subsequent PCR reactions;

2. The preparation of PCR reaction system should be performed in an ice water bath to improve the specificity of amplification.

For detailed information, please check the product manual.