Color All-in-one Reverse Transcription Kit (with DNase)（RT3CP）

Description

The EZBioscience^® Color All-in-one Reverse Transcription Kit (with DNase) is a new generation of rapid reverse transcription kit for genomic DNA removal and reverse transcription reaction in one step. Compared with the EZBioscience^® Color Reverse Transcription Kit (with gDNA Remover) (Cat. No.: A0010CG), this kit does not require a separate genomic DNA removal reaction, which is more convenient and can effectively reduce the risk of contamination and RNA degradation caused by complex sampling process. The kit mainly includes five tubes of reagents: Enzyme Mix, 5× Color All-in-one RT Buffer, NC Buffer, Oligo dT18 (20×) and Random Hexamer (20×). Among them, The Enzyme Mix contains DNase, RNase Inhibitor and reverse transcriptase; The 5× Color All-in-one RT Buffer contains buffer, dNTPs, blue dye, etc.; Oligo dT18 primer and Random Hexamer are packaged separately, which is convenient for adding gene-specific primers as needed.

Blue dye in the 5× Color All-in-one RT Buffer could avoid pipetting errors. It can be used with the EZBioscience^® Color SYBR Green qPCR Mix (Cat. No.: A0012, A0012-R1, A0012-R2), which is supplemented with an inert red dye. Mixing cDNAs, primers and other components with the EZBioscience^® Color SYBR Green qPCR Mix together in a qPCR reaction turns the solution into purple, which provides a visual aid when pipetting and decreases the risk of pipetting errors during reaction setup, especially when using white reaction vessels. And the dyes do not affect the specificity or sensitivity of qPCR reactions. So, these two kinds of kits are recommended to use together to get optimal results.

The reverse transcriptase in the Enzyme Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. The reverse transcription reaction products are recommended for PCR, qPCR and gene cloning.

Components

 Storage

Store at -20°C.

Features

1. Fast and simple procedure: genomic DNA removal and reverse transcription reaction is carried out in one step.

2. The NC Buffer does not contain reverse transcriptase, which can be used to detect whether there is genomic DNA residue in RNA template.

3. 5× Color All-in-one RT Buffer contains blue dye, which can be used with the EZBioscience^® Color SYBR Green qPCR Mix (A0012, A0012-R1, A0012-R2), which is supplemented with an inert red dye. Mixing cDNAs, primers and other components with the EZBioscience^® Color SYBR Green qPCR Mix together in a qPCR reaction turns the solution into purple, which provides a visual aid when pipetting and decreases the risk of pipetting errors during reaction setup, especially when using white reaction vessels.

4. Oligo dT18 and Random Hexamer in this kit are packaged separately, so different types of reverse transcription primers can be used flexibly for different experimental designs.

5. Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, this kit reverses cDNA with the smallest quantitative CT value and has excellent reverse transcription efficiency.

6. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.

For detailed information, please check the product manual.