Cell Viability Detection Kit (Luminescence) (EZB-CV1)

Introduction

The EZBioscience^®  Cell Viability Detection Kit (Luminescence) provides a homogeneous method to detect cell viability or the number of viable cells in culture by  quantitating the amount of ATP present, which indicates the presence of metabolically active cells. This ready-to-use reagent is based on the luciferase reaction, containing thermostable luciferase (luciferase) and high-purity luciferin (luciferin). The homogeneous assay procedure involves addition of ATP Detection Reagent directly  to cells cultured  in serum-supplemented medium to lyse the cells to release ATP without washing the cells and removal of medium. and the reaction shown in the figure below can occur. The reagent has excellent performance, good linear correlation, high specificity, and long signal half-life. At the same time, the ATP Detection Reagent is much more stable and will maintain > 85% light output upon storage at 4°C for several weeks.

Figure 1. Overview of Cell Viability Detection Kit (Luminescence) principle.

Component

Storage

All components should be stored at -20°C and protected from light. For maximum light signal, we recommend long term storage at –80°C. The reagent can withstand four additional freeze-thaw cycles after the first thaw with no loss of light output.

For detailed information, please check the product manual.