EZ-Probe qPCR Master Mix for microRNA (No ROX) (EZB-miProbe-R0)

EZ-Probe qPCR Master Mix for microRNA (No ROX) (EZB-miProbe-R0)

Type:     miRNA RT-qPCR

Catalog Number:    EZB-miProbe-R0-S/EZB-miProbe-R0

Pack Size:    200/500 Rxns

Price:    250/500 USD

Promotion:    180/350 USD (33 Customers Bought)


The EZBioscience® EZ-Probe qPCR Master Mix for microRNANo ROXuses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.




(200 Rxns)


(500 Rxns)

2× EZ-Probe qPCR Master Mix for microRNA

2 ml

5 ml

Probe   (10 μM)

45 µl

110 µl

Universal 3’ qPCR Primer (10 μM)

100 μl

250 μl

U6 Primer (10 μM )

50 μl

125 μl


All components should be stored at -20°C and avoid light and repeated thawing. The Probe is suggested to divide into 3~4 parts, and store at -80°C and avoid light for long time storage. The quality of this product is guaranteed for 12 months under recommended storage conditions.


Appropriate Real-time PCR instruments for this Kit are as follows:

Bio-Rad   CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®,   Opticon 2, Chromo4™; Roche LightCyclerTM 96,   Roche   LightCyclerTM 480; Eppendorf Mastercycler® ep   realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene®   Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo   Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.

For detailed information, please check the product manual.