EZscript All-in-one Reverse Transcription Kit (with DNase)（RT3）
Type: Reverse Transcription Reagents
Catalog Number: RT3 / RT3-L
Pack Size: 100 Rxns / 500 Rxns
Price: 638 USD / 3000 USD
Promotion: 510 USD / 2400 USD
Description
The EZBioscience® EZscript All-in-one Reverse Transcription Kit (with DNase) is a new generation of rapid reverse transcription kit for genomic DNA removal and reverse transcription reaction in one step. Compared with the EZBioscience® 4× EZscript Reverse Transcription Mix II (with gDNA Remover) (Cat. No.: EZB-RT2GQ), this kit does not require a separate genomic DNA removal reaction, which is more convenient and can effectively reduce the risk of contamination and RNA degradation caused by complex sampling process. The kit mainly includes three tubes of reagents: Enzyme Mix containing DNase, RNase inhibitor and reverse transcriptase; 5× EZscript All-in-one RT Buffer, containing buffer, dNTPs, Oligo dT18 and Random Hexamer, etc; NC Buffer.
The reverse transcriptase is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of the reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. The reverse transcription reaction products are recommended for PCR and qPCR.
Components
Components | RT3 (100 Rxns) | RT3-L (500 Rxns) |
Enzyme Mix | 200 μl | 200 μl × 5 tubes |
5× EZscript All-in-one RT Buffer | 400 μl | 400 μl × 5 tubes |
NC Buffer | 20 μl | 20 μl × 5 tubes |
Nuclease free ddH2O | 1 ml | 1 ml × 5 tubes |
Storage
Store at -20°C.
Features
1. Fast and simple procedure: genomic DNA removal and reverse transcription reaction is carried out in one step.
2. The NC Buffer does not contain reverse transcriptase, which can be used to detect whether there is genomic DNA residue in RNA template.
3. Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, this kit reverses cDNA with the smallest quantitative CT value and has excellent reverse transcription efficiency.
4. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.
For detailed information, please check the product manual.
