Description
The EZBioscience® EZ-press RNA Purification Kit PLUS provides a simple, reliable, and rapid method for isolating high-quality RNA from a wide variety of sources, without the need for toxic substances such as phenol or chloroform. The kit can be used with animal cells. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, the lysate is passed through the DNA Removing Spin Column which traps the DNA on the column, while RNA can pass through the column and retain in the flow-through. Ethanol is added to the flow-through, creating conditions that promote selective binding of RNA to the silica membrane. Then the sample is applied to a Spin Column for RNA, where the RNA binds to the membrane. Contaminants are efficiently washed away by subsequent step of washing. The high-quality RNA is then eluted in Elution Buffer and may be used in a variety of downstream applications, including: RT-PCR, RT-qPCR, RNA-seq, Northern blotting and so on.
Components
Components | B0004-plus (100 Preps) |
Lysis Buffer | 55 ml |
Wash Buffer* | 13 ml |
Elution Buffer | 10 ml |
DNA Removing Spin Columns (with Collection Tubes) | 100 Preps |
Spin Columns for RNA (with Collection Tubes) | 100 Preps |
*: Before using for the first time, add 52 ml of 100% ethanol to the Wash Buffer and mix thoroughly.
Storage
Store DNA Removing Spin Columns (with Collection Tubes) at 4 °C；Store other components at room temperature (When using these buffers, be careful to avoid of contamination).
Experimental Procedure at a Glance
Features
1. Fast and simple procedure delivering high-quality total RNA in ~8 minutes.
2. Efficient gDNA removal with unique DNA Removing Spin Columns.
3. Safe and non-toxic: no phenol/chloroform extraction, or ethanol precipitation.
4. High-quality RNA for downstream applications: RT-PCR, RT-qPCR, RNA Sequencing, and so on.
Representative Experimental Results
Figure 1. RNA isolated from A549 cells using EZBioscience® EZ-press RNA Purification Kit PLUS was detected with Nanodrop (eluted in 30 μl of Elution Buffer). The result showed that high quality RNA can be isolated by EZBioscience® EZ-press RNA Purification Kit PLUS.
Figure 2. RNA isolated from A549 cells using EZBioscience® EZ-press RNA Purification Kit PLUS and TRIzol (eluted in 30 μl of Elution Buffer each, 5 μl RNA of each is loaded for electrophoresis). M: 250bp DNA Ladder; Lane 1: TRIzol; Lane 2: EZBioscience® EZ-press RNA Purification Kit PLUS. The result showed that high quality RNA can be isolated by EZBioscience® EZ-press RNA Purification Kit PLUS.
For detailed information, please check the product manual.
