Description
The EZBioscience® 4× EZscript Reverse Transcription Mix II is a new-generation reverse transcription kit with higher reverse transcription efficiency compared to previous generations.
The kit is a ready-to-use premixed reagent that contains all the components necessary to synthesize first strand cDNA from total or poly(A)+ RNA using reverse transcriptase. Reaction products are applicable to subsequent PCR, qPCR. The mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs; Oligo dT18 and Random Hexamer as primer. The random primer in the Mix can help get better Ct values.
The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. The reverse transcriptase lacking RNase H activity is suitable for preparing full-length cDNA. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this reverse transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.
When using this kit, 4× EZscript RT Mix II should be added to total RNA template, as recommended in the protocol, and add ddH2O to a total volume of 20 μl. Then, mix well and run the RT reaction.
Components
Components | EZB-RT2 (100 Rxns) | EZB-RT2-L (500 Rxns) |
4× EZscript RT Mix II | 550 μl | 550 μl × 5 tubes |
Nuclease free ddH2O | 1 ml | 1 ml × 5 tubes |
Storage
Store at -20°C.
Features
1. Fast and simple procedure: 4× EZscript RT Mix II contains all the components required for reverse transcription reaction, only need to add template RNA, the reverse transcription reaction can be completed in 20 minutes.
2. Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, this kit reverses cDNA with the smallest quantitative CT value and has excellent reverse transcription efficiency.
3. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.
For detailed information, please check the product manual.
