EZ-Probe qPCR Master Mix for microRNA (ROX2 plus) (EZB-miProbe-R2)

EZ-Probe qPCR Master Mix for microRNA (ROX2 plus) (EZB-miProbe-R2)

Type:     miRNA RT-qPCR

Catalog Number:    EZB-miProbe-R2-S/EZB-miProbe-R2

Pack Size:    200/500 Rxns

Price:    250/500 USD

Promotion:    180/350 USD (47 Customers Bought)


The EZBioscience® EZ-Probe qPCR Master Mix for microRNAROX2 plususes a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.




(200 Rxns)


(500 Rxns)

2× EZ-Probe qPCR Master Mix for microRNA (ROX2 plus)*

2 ml

5 ml

Probe   (10 μM)

45 µl

110 µl

Universal 3’ qPCR Primer (10 μM)

100 μl

250 μl

U6 Primer (10 μM )

50 μl

125 μl

*: The ROX2 premixed is used to rectify the error of fluorescence signals between different wells.


All components should be stored at -20°C and avoid light and repeated thawing. The Probe is suggested to divide into 3~4 parts, and store at -80°C and avoid light for long time storage. The quality of this product is guaranteed for 12 months under recommended storage conditions.


Appropriate Real-time PCR instruments for this Kit are as follows:

Bio-Rad CFX96™,   CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon   2, Chromo4™; Roche LightCyclerTM   96,   Roche LightCyclerTM   480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina   Eco qPCR; Qiagen/Corbett   Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene®   6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.

ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7;   Stratagene MX3000P™, MX3005P™, MX4000™

For detailed information, please check the product manual.