EZ-Probe qPCR Master Mix for microRNA (ROX2 plus) (EZB-miProbe-R2)

Introduction

The EZBioscience^® EZ-Probe qPCR Master Mix for microRNA（ROX2 plus）uses a specially modified Taq DNA polymerase protected by hot-start activation technique, and specifically optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR) for microRNAs detection. The mix is prepared at 2× reaction concentration. Meanwhile, it provides a tube of appropriative dedicated probe, Universal 3’ qPCR Primer, and U6 Primer, which is well matched with the cDNA obtained by the several microRNA Reverse Transcription Kits from the EZBioscience^® (Cat. No.: EZB-miRT1, EZB-miRT4). So, it allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses for microRNAs.

Components

*: The ROX2 premixed is used to rectify the error of fluorescence signals between different wells.

Storage

All components should be stored at -20°C and avoid light and repeated thawing. The Probe is suggested to divide into 3~4 parts, and store at -80°C and avoid light for long time storage. The quality of this product is guaranteed for 12 months under recommended storage conditions.

Notice

Appropriate Real-time PCR instruments for this Kit are as follows:

For detailed information, please check the product manual.