EZProbe One Step qRT-PCR Probe Kit

Introduction

The EZBioscience^® EZProbe One Step qRT-PCR Kit is a one-step kit to quantify RNA using fluorescent probe. With the exception of primers, probe and template, this kit contains in four vials all the components required to perform rapid, sensitive and reproducible qRT-PCR.

20× EZProbe One Step Enzyme Mix contains M-MLV (RNase H-) reverse transcriptase, RNase Inhibitor and Hot Start DNA Polymerase; 2× EZProbe One Step Buffer contains dNTPs, Oligo dT18, Random Hexamer and optimized buffer; 50× ROX Reference Dyes are used to rectify the error of fluorescence signals between different wells.

The M-MLV Reverse Transcriptase, which is an RNA-dependent DNA polymerase with a significantly attenuated RNase H activity, can synthesize cDNA at a temperature range of 42°C to 60°C and is inactivated during the activation step of the Hot Start DNA Polymerase.

RNase inhibitor can protect RNA templates from degradation.

This buffer has been optimized to allow both reverse transcription and PCR amplification to occur in the same reaction across a wide range of templates.

Hot Start DNA Polymerase requires an activation step at 95°C for 5 minutes, which prevents non-specific amplification during cDNA synthesis.

Components

*1: Contain reverse transcriptase, RNase Inhibitor, Hot Start DNA Polymerase.

*2: Contain dNTPs, Oligo dT18, Random Hexamer and optimized buffer.

*3: Used to rectify the error of fluorescence signals between different wells.

Storage

The kit should be stored at -20°C and protected from light. Avoid repeated freeze thawing. The quality of this product can be guaranteed for 18 months under recommended storage conditions.

Notice

Please select an appropriate ROX according to the Real-time PCR instrument used:

Representative Results

Figure1. GAPDH mRNA expression level in serially diluted RNA from SARS-CoV-2-pseudovirus were detected. The amplification plots above indicate that the target gene could be amplified efficiently and specifically.

For detailed information, please check the product manual.