Description
The EZ-Press 96 RNA Purification Kit is an ideal reagent kit for simultaneously isolating 96 or 192 RNA samples from 2 × 104 to 5 × 105 human or animal cells. The EZ-Press 96 RNA Purification Kit provides an efficient and high-throughput solution for RNA sample preparation in areas such as drug screening, treatment monitoring, and basic research. In less than 1 hour, when processing two 96-well RNA plates in parallel, up to 192 high-purity RNA samples can be obtained (approximately 20 seconds per RNA sample).
The EZ-Press 96 RNA Purification Kit replaces current time-consuming and complex methods, including ethanol precipitation, extensive washing steps, or the use of toxic substances such as phenol and chloroform. The purified RNA can be used for various downstream applications. Including cDNA synthesis, RT-PCR, RT-qPCR, Northern, dot, and slot blot analysis, as well as primer extension, RNase/S1 nuclease protection, and gene chip detection. In addition, the EZ-Press 96 RNA Purification Kit can be used for purifying RNA from enzyme-catalyzed reactions, such as DNA digestion, protein digestion, RNA ligation, labeling reactions, etc.
Components
Components
| EZ4001-S (2 × 96 Preps) | EZ4001-L (12 × 96 Preps) |
96-Well RNA Plates*1 | 2 | 12 |
Caps for Elution Plates*1 | 2 | 12 |
Micro-pore Tape Sheets*1 | 4 | 24 |
96-Well Collection Plates (Square Hole) *1 | 2 | 12 |
96-Well Elution Plates (Round Hole) *1 | 2 | 12 |
Buffer RLB | 80 ml | 240 ml × 2 |
Buffer RW1*2 | 70 ml | 210 ml × 3 |
Buffer RW2*2 | 30 ml | 90 ml × 3 |
Elution Buffer*3 | 30 ml | 30 ml × 8 |
Note: *1. For the sake of simplicity, these 5 devices will be referred to as RNA extraction well plate, plate lid, breathable sealing film/sealing film, collection well plate, and elution well plate in this article. *2. Before using Buffer RW1 and Buffer RW2 for the first time, it is necessary to add the volume of anhydrous ethanol indicated on the bottle label (the volume ratio of Buffer RW1 to anhydrous ethanol is 1:1, and the volume ratio of Buffer RW2 to anhydrous ethanol is 1:4), and mix thoroughly before use. *3. The Elution Buffer in this reagent kit is Nuclease-free ddH2O.
Storage
All components should be stored at room temperature and can be stably stored for 12 months. See the expiration date on the product label for details.
Notice
1. Before using Buffer RW1 and Buffer RW2 for the first time, it is necessary to add the volume of anhydrous ethanol indicated on the bottle label (the volume ratio of Buffer RW1 to anhydrous ethanol is 1:1, and the volume ratio of Buffer RW2 to anhydrous ethanol is 1:4), and mix thoroughly before use.
2. Regarding centrifugation: all centrifugation operations with the well plates require placing the 96-Well RNA Plates (hereinafter referred to as "RNA extraction plate") on the 96-Well Collection Plates (hereinafter referred to as "collection plate") or the 96-Well Elution Plates (hereinafter referred to as "elution plate") as needed, after adding the liquid, cover the RNA extraction plate with a Micro-pore Tape Sheet (hereinafter referred to as "breathable sealing film/sealing film") before centrifugation. The collection plate needs to be reused during the cleaning stage, and a separate elution plate should be used during the elution stage.
3. The recommended range for the cell counts of each sample is 2 × 104 to 5 × 105.
4. For your safety and health, please wear a lab coat, disposable latex gloves, and disposable masks.
5. The total RNA extracted by this kit may contain a very small amount of genomic DNA, which will not significantly affect the subsequent reverse transcription and qPCR (especially when the reverse transcription step includes a process to remove genomic DNA, or when the qPCR primers are designed to span intron). For experiments that are highly sensitive to residual genomic DNA, it is recommended to use the EZ4002 kit (which includes a dedicated genomic DNA removal plate that can effectively remove genomic DNA).
Materials
Tabletop centrifuge with swing-out rotor for deep-well plates at a speed of 2100 g or higher, anhydrous ethanol, horizontal shaker, etc.
For detailed information, please check the product manual.
