The EZBioscience® Total RNA Extraction Reagent is a ready-to-use reagent, which designs to isolate high quality total RNA from animal tissues, cultured cells, plants, yeast, bacteria and other samples within 1 hour. Total RNA Extraction Reagent is a monophasic solution of phenol, guanidine isothiocyanate and other components, which maintains the integrity of the RNA due to highly effective inhibition of RNase activity during sample homogenization. After homogenizing the sample with Total RNA Extraction Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA, polysaccharide, fatty acid, etc). RNA is precipitated from the aqueous layer with isopropanol. The precipitated RNA is washed to remove impurities, and then resuspended for use in downstream applications. Isolated RNA can be used in RT-PCR, Northern Blot analysis, Dot Blot hybridization, poly(A)+ selection, in vitro translation and molecular cloning.
Total RNA Extraction Reagent
Store the Total RNA Extraction Reagent at 2 ~ 8°C, protect from light.
For detailed information, please check the product manual.