2× EZ SYBR Green qPCR Master Mix (Q23)

2× EZ SYBR Green qPCR Master Mix (Q23)

  • Type:     Realtime-PCR Reagents

  • Catalog Number:    Q23

  • Pack Size:    5ml 

  • Price:    200 USD

  • Promotion:    160 USD (156 Customers Bought)


The EZBioscience®2× EZ SYBR Green qPCR Master Mix is a kit specifically designed for intercalator-based real-time PCR using SYBR Green I for detection. The Premix is supplied in 2× concentration and contains SYBR Green I, dNTPs, optimized buffer and a genetically engineered hot-start Taq DNA polymerase which uses an anti-Taq antibody, making it easy to prepare reaction mixtures. In addition, this kit contains 50× ROX Reference Dye 1 (ROX1) and 50× ROX Reference Dye 2 (ROX2), which are suitable for all qPCR instruments. This kit allows high amplification efficiency and high detection sensitivity in real-time PCR. 





2× EZ SYBR Green qPCR Master Mix*1

5 ml

25 ml

50× ROX Reference Dye 1

220 µl

220 µl × 5 tubes

50× ROX Reference Dye 2

220 µl

220 µl × 5 tubes

*1: Contain a hot-start PCR enzyme with an anti-Taq antibody, dNTPs, Mg2+, buffer, and SYBR Green I.

*2: Used to rectify the error of fluorescence signals between different wells.


All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under the recommended storage conditions.

Compatible instrument systems

Please select an appropriate ROX according to the real-time PCR instrument used:

Do Not Use ROX

Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™ 5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Roche LightCycler™ 96, Roche LightCycler™ 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.

Use ROX1 

ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.

Use ROX2

ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™.

For detailed information, please check the product manual.