EZ-Probe qPCR Master Mix-UDG（EZB-Probe-U）
Type: Realtime-PCR (Probe-based qPCR)
Catalog Number: EZB-Probe-U / EZB-Probe-U-L
Pack Size: 5 ml / 25 ml
Price: 638 USD / 3000 USD
Promotion: 425 USD / 2000 USD
Introduction
The EZBioscience® EZ-Probe qPCR Master Mix-UDG uses a specially modified Taq DNA polymerase protected by Hot Start technique, and optimized qPCR buffer system to perform probe based real-time quantitative PCR (qPCR). The Mix is supplied at 2× concentration, which allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses. In addition, the Mix contains Uracil-DNA N-Glycosylase (UDG) which can prevent carryover contamination in PCR and ensure the accuracy of the results.
Components
Components | EZB-Probe-U (500 Rxns) | EZB-Probe-U-L (2,500 Rxns) |
2× EZ-Probe qPCR Master Mix-UDG*1 | 5 ml | 25 ml |
50× ROX Reference Dye 1*2 | 220 µl | 220 µl × 5 tubes |
50× ROX Reference Dye 2*2 | 220 µl | 220 µl × 5 tubes |
*1: primers/probe is not supplied in this product; it contains UDG, dNTP blend containing dUTP/dTTP, Hot Start DNA Polymerase, optimized buffer.
*2: Used to rectify the error of fluorescence signals between different wells.
Storage
All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under recommended storage conditions.
Notice
Please select an appropriate ROX according to the real-time PCR instrument used:
Do Not Use ROX | Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™, Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®. |
Use ROX 1 (1×) | ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™. |
Use ROX 2 (1×) | ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™. |
Representative Results
Figure1. GAPDH, BAIAP2L2, FIBRONECTIN and P53 mRNA expression level in cDNA from A549 cells were detected. The amplification plots above indicate that, all of the four genes could be amplified efficiently and specifically (Notes: Quantitation is fine when Ct value is within 13 ~ 32; if the Ct value is larger than 35, the amplification is invalid; If the Ct value is too small (＜13), please dilute the template and repeat the quantitation; if the Ct value is within 33 ~ 35, 3 replicates are required to validate the results).
Figure2. GAPDH mRNA expression level in serially diluted cDNAs from A549 cells were detected. The amplification plots above indicate that, the target gene could be amplified efficiently and specifically. And the linear relationship within the detection range is very good.
For detailed information, please check the product manual.
