EZ-Probe qPCR Master Mix (EZB-Probe)
Type: Realtime-PCR (Probe-based qPCR)
Catalog Number: EZB-Probe/EZB-Probe-L
Pack Size: 500/2500 Rxns
Price: 300/1500 USD
Promotion: 210/1000 USD (35 Customers Bought)
Introduction
The EZBioscience® EZ-Probe qPCR Master Mix uses a specially modified Taq DNA polymerase protected by Hot Start technique, and optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR). The mix is supplied at 2× concentration, and allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses.
Components
Components | EZB-Probe (500 Rxns) | EZB-Probe-L (2,500 Rxns) |
2× EZ-Probe qPCR Master Mix*1 | 5 ml | 25 ml |
50× ROX Reference Dye 1*2 | 220 µl | 220 µl × 5 tubes |
50× ROX Reference Dye 2*2 | 220 µl | 220 µl × 5 tubes |
*1: Primers/probe is not supplied in this product.
*2: Used to rectify the error of fluorescence signals between different wells.
Storage
All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under recommended storage conditions.
Notice
Please select an appropriate ROX according to the Real-time PCR instrument used:
Do Not Use ROX | Bio-Rad CFX96™, CFX384™, iCycler iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM 480; Eppendorf Mastercycler® ep realplex, realplex 2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Thermo Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®. |
Use ROX 1 (1×) | ABI 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™. |
Use ROX 2 (1×) | ABI 7500, 7500 Fast, Quant-Studio 3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™, MX4000™. |
For detailed information, please check the product manual.
