EZ-Probe qPCR Master Mix (EZB-Probe)

EZ-Probe qPCR Master Mix (EZB-Probe)

Type:     Realtime-PCR (Probe-based qPCR)

Catalog Number:    EZB-Probe/EZB-Probe-L

Pack Size:    500/2500 Rxns

Price:    300/1500 USD

Promotion:    210/1000 USD (35 Customers Bought)


The EZBioscience® EZ-Probe qPCR Master Mix uses a specially modified Taq DNA polymerase protected by Hot Start technique, and optimized qPCR buffer system to perform Probe based Real-time quantitative PCR (qPCR). The mix is supplied at 2× concentration, and allows accurate target quantification and detection over a broad dynamic range and makes it possible to conduct highly reproducible and reliable qPCR analyses.




(500 Rxns)


(2,500 Rxns)

2× EZ-Probe qPCR Master Mix*1

5 ml

25 ml

50× ROX Reference Dye 1*2

220 µl

220 µl × 5 tubes

50× ROX Reference Dye 2*2

220 µl

220 µl × 5 tubes

*1: Primers/probe is not supplied in this product.

*2: Used to rectify the error of fluorescence signals between different wells.


All components should be stored at -20°C and protected from light. The quality of this product can be guaranteed for 18 months under recommended storage conditions.


Please select an appropriate ROX according to the Real-time PCR instrument used:

Do Not   Use ROX

Bio-Rad CFX96™, CFX384™, iCycler   iQ™, iQ™5, MyiQ™, MiniOpticon™,Opticon®, Opticon 2, Chromo4™; Roche LightCyclerTM 96, Roche LightCyclerTM   480; Eppendorf Mastercycler® ep realplex, realplex   2s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000,   Rotor-Gene® 6000; Thermo   Scientific PikoReal Cycler; Analytikjena qTOWER 3G; Cepheid SmartCycler®.

Use ROX 1 (1×)

ABI 5700,   7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.

Use ROX 2 (1×)

ABI 7500, 7500 Fast, Quant-Studio   3, 5, 6, 7, 12K Flex, Dx, ViiA™7; Stratagene MX3000P™, MX3005P™,   MX4000™.

For detailed information, please check the product manual.